Reporter

Part:BBa_K1891011:Design

Designed by: Zhao Jingyu   Group: iGEM16_BNU-China   (2016-10-10)


beta-tubulin+YCE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 248
    Illegal BamHI site found at 1031
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 289
    Illegal AgeI site found at 97
    Illegal AgeI site found at 427
    Illegal AgeI site found at 1195
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We connected this two gene fragments using fusion PCR. First we acquired beta-tubulin gene fragment and YCE gene fragment. And then we redesigned a antisense primer of beta-tubulin and a sense primer of YCE with a linker about 42bp. And then doing fusion PCR.


Source

Beta-tubulin comes from human breast cancer cell line Mcf-7. YCE comes from pSPYCE plasmid.

References